East Tennessee State University
Measuring the Changes in Tumor Necrosis Factor-Alpha (TNF-α) from Secretory Populations of U937 Monocytic Cells during Differentiation.
Abstract
dc:description.abstract<p>Tumor necrosis factor-alpha (TNF-α) is a cytokine produced primarily by macrophages during acute inflammation. In this study we examined the differential effect of retinoic acid (RA) and phorbol 12-myristate 13-acetate (PMA) on the induction of TNF-α secretion from U937 monocytic cell populations by using the reverse hemolytic plaque assay (RHPA). The RHPA will allow us to investigate both changes in TNF-α secreting populations as well as monitor the relative amount of TNF-α released from individual cells. Our results indicate that treatment of U937 cells with RA (10<sup>-6</sup>M) moderately increases the secreting cell populations, and dramatically enhances the amount of TNF-α secreted from cells already committed to secretion. In contrast, treatment with PMA (250ng/ml) drastically increased the secreting population, but only slightly increasing the amount of TNF-α released. These results suggest that induction of TNF-α secretion from U937 cells occurs by different pathways.</p>
Degree
thesis:*- Name thesis:degree_name
- MS (Master of Science)
- Level thesis:degree_level
- Thesis - restricted
- Discipline thesis:degree_discipline
- Biology
- Year dc:date.issued
- 2002
Author and committee
dc:creator, dc:contributor.*- Author dc:creator
-
- Tran, An Xuong
Subjects
dc:subject × 6Rights
dc:rights- Statement dc:rights
-
- Copyright by the authors.
Identifiers
dc:identifier.*- Repository record dc:identifier
- https://dc.etsu.edu/etd/685
- OAI identifier oai:identifier
- oai:dc.etsu.edu:etd-1842