Characterization of a Novel Water Stress Related Protein from Geranium (<em>Pelargonium hortorum</em>).

<p>Searches for sequence similarity against a previously isolated and known ABA regulated partial geranium cDNA on GenBank and TIGR databases gave results indicating sequence similarities to both firefly luciferase and 4-coumarate:CoA Ligase (4-CL). To determine whether or not this geranium cDNA, and a root mRNA from <em>Arabidopsis</em> that hybridized with the geranium cDNA, is 4-CL, specific <em>Arabidopsis</em> 4-CL primers were used for expression analysis by RT-PCR of <em>Arabidopsis</em> 4-CL mRNA from <em>Arabidopsis</em> root tissue. The expression pattern of the <em>Arabidopsis</em> 4-CL mRNA was identical with the expression pattern of the mRNA that hybridized with the geranium cDNA. This matching expression pattern gives very strong evidence that our geranium cDNA is 4-CL like and that <em>Arabidopsis</em> 4-CL is downregulated during water stress by ABA. This is significant in that it has not previously been reported. Northern analysis and PCR product sequence analysis further confirmed this expression data.</p>

Name thesis:degree_name

MS (Master of Science)

Level thesis:degree_level

Thesis - restricted

Discipline thesis:degree_discipline

Biology

Year dc:date.issued

2001

Author dc:creator

• Ashley, David W

• abscisic acid
• water stress
• lignin
• RT-PCR
• phenylpropanoid
• Biology
• Life Sciences

Statement dc:rights

• Copyright by the authors.

Repository record dc:identifier

https://dc.etsu.edu/etd/39

OAI identifier oai:identifier

oai:dc.etsu.edu:etd-1089