The effect of the reducing agent triphenylphosphine on arachidonic acid metabolism by prostaglandin H2 synthase

<p>Prostaglandin H2 synthase (PGHS) catalyzes the conversion of arachidonic acid to prostaglandin endoperoxide (PGH2), which is a precursor to a variety of prostaglandins that function as hormones. Gas chromatography combined with mass spectrometry is employed to look for the novel products produced by reaction of triphenylphosphine with arachidonic acid prior to and during the PGHS reaction. Triphenylphosphine (TPP) is known to reduce hydro peroxides to alcohols and is used in the present study to ascertain the extent to which hydroperoxides in arachidonic acid preparations and in PGHS contribute to enzyme activity. Incubation of PGHS with arachidonic acid that was treated with triphenylphosphine resulted in two peaks. One of the peaks was identified as Prostaglandin E2, the well-characterized metabolite of PGHS activity. PGHS metabolism of arachidonic acid in the presence of TPP resulted in production of a new metabolite that was identified as 12-hydroxyeicosatetraenoic acid.</p>

Name thesis:degree_name

Master of Arts (MA)

Level thesis:degree_level

Open Access Thesis

Discipline thesis:degree_discipline

Chemistry

Year

2007

Author dc:creator

• Ailaboina, Lalini

• Pharmaceutical chemistry
• Prostaglandins Synthesis
• Arachidonic acid Metabolism
• Chemistry

Repository record dc:identifier

https://commons.emich.edu/theses/19

OAI identifier oai:identifier

oai:commons.emich.edu:theses-1018