A Peptide-Based Platform for Displaying Antibodies to Engage T Cells

This study investigated a strategy by which antibodies were displayed on a gel-like substance to engage T cells. The substance, a peptidic composite, was characterized in vitro and explored as an injectable system in vivo. The composite consists of two amphiphilic peptides, AEAEAKAKAEAEAKAK (referred to as "EAK") and AEAEAKAKAEAEAKAKHHHHHH ("EAKH6"). Spectroscopic analysis showed the two peptides integrated into a single structure. Prior to combination, conformational analysis revealed that EAKH6 adopts a mixed alpha-helix/bata-strand conformation. In the presence of EAK, EAKH6 exists predominantly in a beta;-strand conformation. Using nickel-bound horseradish peroxidase as a probe, the composite of EAK-EAKH6 was found to display His-tags. T-cell-specific antibodies were found stably displayed on the EAK-EAKH6 assembly using recombinant protein A/G and anti-hexahistidine antibody as an adaptor. When mounted with an anti-CD4 antibody, the system was shown to capture CD4 T cells in a mixed population of lymphocytes. Antibodies were concentrated in the subcutaneous space in mice when co-administered with EAK and EAKH6 along with protein A/G and anti-hexahistidine antibody as an aqueous (deionized water)

Name thesis:degree_name

PhD

Level thesis:degree_level

Immediate Access

Discipline thesis:degree_discipline

Pharmaceutics

Year dc:date.available

2010

Author dc:creator

• Zheng, Ying

Contributors dc:contributor

• Wilson Meng
• James Drennen
• Philip Auron
• Ellen Gawalt
• Peter Wildfong

• EAK
• Histidine
• Insoluble structure
• Monoclonal antibody
• Regulatory T cells
• Self-assembling peptides

Language dc:language

English

Repository record dc:identifier

https://dsc.duq.edu/etd/1412

OAI identifier oai:identifier

oai:dsc.duq.edu:etd-2428