Transgenerational Plasticity is Sex-dependent and Persistent in Yellow Moneyflower (Mimulus guttatus)

Transgenerational phenotypic plasticity, whereby environmental cues experienced by parents alter the phenotype of their progeny, has now been documented in diverse organisms. Transmission of environmentally determined responses is known to occur through both maternal and paternal gametes, but the underlying mechanisms have rarely been compared. In addition, the persistence of induction over multiple generations appears to vary widely but has been characterized for relatively few systems. Yellow monkeyflower (<em>Mimulus guttatus</em>) is known to exhibit transgenerational induction of increased glandular trichome production in response to simulated insect damage. Here we test for differences between maternal and paternal transmission of this response and examine its persistence over five generations following damage. Maternal and paternal damage resulted in similar and apparently additive increases in progeny trichome production. Treatment of germinating seeds with the genome-wide demethylating agent 5-azacytidine erased the effect of maternal but not paternal damage. The number of glandular trichomes remained elevated for three generations following damage. These results indicate that transgenerational transmission occurs through both maternal and paternal germ lines, but that they differ in the proximate mechanism of epigenetic inheritance. Our results also indicate that a wounding response can persist for multiple generations in the absence of subsequent damage.

Name thesis:degree_name

Master of Science (MS)

Discipline thesis:degree_discipline

Biology

Year dc:date.available

2019

Author dc:creator

• Akkerman, Kayla

Contributors dc:contributor

• Alison G. Scoville
• Jennifer Dechaine
• Mary E. Poulson

• Evolution
• Biology
• Epigenetics
• Other Ecology and Evolutionary Biology

Language dc:language

English

Repository record dc:identifier

https://digitalcommons.cwu.edu/etd/1268

OAI identifier oai:identifier

oai:digitalcommons.cwu.edu:etd-2286