The Chemical Analysis and Biological Activities of the Secondary Metabolites from Dalea mollis and Dalea albiflora

Multidrug resistance has increased since the introduction of drugs used to prevent growth and kill microorganisms in a host. This has caused a worldwide search to discover new drugs effective against microorganisms. Mechanisms of drug resistance include, but are not limited to, the production of biofilms and efflux pumps. Efflux pumps prevent antimicrobial drugs from reaching their biological target, so the coordinated use of efflux pump inhibitors and antimicrobial drugs has been identified as a potential treatment for multidrug-resistant microorganisms. The secondary metabolites of <em>Dalea mollis </em>and <em>Dalea albiflora</em> were tested against multidrug-resistant (MDR) and engineered strains of the fungi <em>Candida glabrata </em>and <em>Saccharomyces cerevisiae</em>. Two known pterocarpans, two known flavanones, and an isoflavonoid were isolated and identified from <em>D. mollis</em>. Two new flavanones (albifloran A <strong>9</strong> and albifloran B <strong>10</strong>), one known pterocarpan, and three known flavanones were isolated and identified from <em>D. albiflora</em>. Initial results revealed that the components of <em>D. mollis</em> were inactive against the fungal strains tested, but components of the roots of <em>D. albiflora</em> did show activity. VLC fractions three and four of <em>D. albiflora</em> inhibited the growth of the <em>S. cerevisiae</em> expression host and <em>S. cerevisiae</em> overexpressing Snq2 with minimal inhibitory concentrations (MIC) of 42 μg/mL and 87 μg/mL, respectively. VLC fraction four inhibited the growth of <em>C. glabrata</em> overexpressing Snq2 with an MIC of 167 μg/mL.

Name thesis:degree_name

Master of Science (MS)

Discipline thesis:degree_discipline

Chemistry

Year dc:date.available

2019

Author dc:creator

• Hansen, Nicholas Peter

Contributors dc:contributor

• Gil Belofsky
• Levente Fabry-Asztalos
• JoAnn Peters

• Chromatography
• Nuclear Magnetic Resonance
• Natural Products
• Organic Chemicals
• Organic Chemistry

Language dc:language

English

Repository record dc:identifier

https://digitalcommons.cwu.edu/etd/1197

OAI identifier oai:identifier

oai:digitalcommons.cwu.edu:etd-2196