Evaluating oxalate-degrading Lactobacillus spp. for their ability to be used as probiotics in the treatment of kidney stone disease

Although the direct cause of kidney stone formation is not known, reports have suggested it is probably a multifactorial disease. Lactobacillus strains which potentially had increased ability to degrade oxalate were previously isolated from a healthy low kidney stone risk group. The aim of this study was to identify these natural Lactobacillus strains and evaluate their potential for use as probiotics in reducing the risk of kidney stone disease. Identification was achieved by PCR amplification and sequencing of the 16S rRNA gene and the 16S-23S rRNA internal transcribed spacer (ITS) region. The strains were identified as follows; Lactobacillus gasseri 7(3), L. gasseri 17(4), Lactobacillus reuteri 17(7) and L. reuteri 16(9). Their probiotic characteristics were also evaluated.

Grantor dc:publisher.institution

Department of Molecular and Cell Biology

Year dc:date.issued

2010

Author dc:creator

• Kabanda, Siti M

Advisors dc:contributor.advisor

• Abratt, Valerie Rose
• Reid, Sharon J

Language dc:language.iso

eng

Handle dc:identifier.uri

http://hdl.handle.net/11427/11460

OAI identifier oai:identifier

oai:open.uct.ac.za:11427/11460