Exploring the Physiological Role of Vibrio fischeri PepN

<p>The primary contributor to Vibrio fischeri aminopeptidase activity is aminopeptidase N, PepN. Colonization assays revealed the pepN mutant strain to be deficient at forming dense aggregates and populating the host’s light organ compared to wildtype within the first 12 hours of colonization; however the mutant competed normally at 24 hours. To address the role of PepN in colonization initiation and establish additional phenotypes for the pepN mutant strain, stress response and other physiological assays were employed. Marked differences were found between pepN mutant and wildtype strain in response to salinity, acidity, and antibiotic tolerance. This study has provided a foundation for future work on identifying a putative role for V. fischeri PepN in regulating stress response.</p>

Name thesis:degree_name

MS in Biological Sciences

Discipline thesis:degree_discipline

Biological Sciences

Year dc:date.available

2015

Author dc:creator

• Cello, Sally L

Contributors dc:contributor

• Pat Fidopiastis

• Aminopeptidase N
• metalloproteases
• Vibrio fischeri
• symbiosis
• Euprymna scolopes
• Bacteriology
• Environmental Microbiology and Microbial Ecology
• Microbial Physiology
• Organismal Biological Physiology
• Other Microbiology

Identifier

10.15368/theses.2015.36

OAI identifier oai:identifier

oai:digitalcommons.calpoly.edu:theses-2602