Single Cell Impedance Measurements Using Microfabricated Electrodes and Labview Graphical Programming

This Master’s Thesis project consists of the research, design, and fabrication of a system that could perform broadband impedance measurements (1kHz-20Mhz) of single cells using National Instruments Labview data acquisition and programming in coordination with a single cell capture device. Presented first is the background information on cells and their electrical properties, along with background in micro-total-analysis systems as well as impedance spectroscopy. Experimental Methods are then discussed for the electrode design, cellular modeling in COMSOL, fabrication methods, and Labview 8.0 Set-up and programming. Measurements were performed using the single-cell capture device on saline, yeast cells, and a polysterene bead. Analysis of the impedance data showed a clear visual and statistically significant difference between live yeast, the bead, and saline. A comparison of live yeast cells to nutrient-starved yeast cells was also performed and a distinct difference in spectra was observed.

Discipline thesis:degree_discipline

Biomedical and General Engineering

Year dc:date.available

2009

Author dc:creator

• Hernandez, Stephanie Sophia

Contributors dc:contributor

• David S. Clague

• Single-cell analysis
• Impedance Spectroscopy
• µ-Tas
• Lab-on-a-chip
• Biomedical Devices and Instrumentation

Identifier

10.15368/theses.2009.192

OAI identifier oai:identifier

oai:digitalcommons.calpoly.edu:theses-1232