Alma Mater Studiorum - Università di Bologna
From "wet biology" to statistical analysis of structural features with bioinformatics tools
Abstract
dc:descriptionMany new Escherichia coli outer membrane proteins have recently been identified by proteomics techniques. However, poorly expressed proteins and proteins expressed only under certain conditions may escape detection when wild-type cells are grown under standard conditions. Here, we have taken a complementary approach where candidate outer membrane proteins have been identified by bioinformatics prediction, cloned and overexpressed, and finally localized by cell fractionation experiments. Out of eight predicted outer membrane proteins, we have confirmed the outer membrane localization for five—YftM, YaiO, YfaZ, CsgF, and YliI—and also provide preliminary data indicating that a sixth—YfaL—may be an outer membrane autotransporter.
Degree
thesis:*- Grantor dc:publisher
- Alma Mater Studiorum - Università di Bologna
- Year dc:date
- 2008
Author and committee
dc:creator, dc:contributor.*- Author dc:creator
-
- Marani, Paola <1970>
- Contributors dc:contributor
-
- Casadio, Rita
Subjects
dc:subject × 1Rights
dc:rights- Statement dc:rights
-
- info:eu-repo/semantics/openAccess
- Language dc:language
- en
Identifiers
dc:identifier.*- Identifier
- urn:nbn:it:unibo-659
- OAI identifier oai:identifier
- oai:amsdottorato.cib.unibo.it:689