Characterization of α₂-adrenergic receptor localization and functional responses

The three α₂-adrenergic receptors (α₂-ARs) were studied in both recombinant and endogenous systems. It was my general hypothesis that the three α₂-adrenergic receptor (AR) subtypes exhibit differential tissue and subcellular localization and couple to physiological pathways to directly elicit functional responses. It was our hope, that in identifying differences between the α₂-ARs we may elucidate their physiological roles and provide a potential means for the rational development of therapeutic drugs or models of such pathologies as atherosclerosis, hypertension and glaucoma. Subtype selective antibodies have been generated and these have provided a tool with which to study the receptors on a molecular and cellular level. Utilizing a number of biochemical, pharmacological, molecular and cellular techniques, I have identified differences between the three receptors with respect to the tissues in which they are expressed, the subcellular domains in which they localize and their patterns of sequestration and down-regulation. In addition, I have demonstrated the expression and co-localization of the α₂-ARs in a variety of native tissues, and a number of functional responses these receptors mediate. These functional responses may have ramifications relevant to tissue healing mechanisms as well as may play a role in a number of pathologies.

Name thesis:degree_name

Ph.D.

Level thesis:degree_level

doctoral

Discipline thesis:degree_discipline

Graduate College

Grantor dc:publisher

The University of Arizona.

Year dc:date.issued

1998

Author dc:creator

• Richman, Jeremy Golding, 1970-

Advisor dc:contributor.advisor

• Regan, John W.

• Biology, Molecular.
• Health Sciences, Pharmacology.
• Biology, Animal Physiology.

Statement dc:rights

• Copyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.

Language dc:language.iso

en_US

Handle dc:identifier.uri

http://hdl.handle.net/10150/282583

OAI identifier oai:identifier

oai:repository.arizona.edu:10150/282583