Proteomsignatur mittels MS-Serumprofiling zur Detektion der Early-onset-Präeklampsie

Preeclampsia is a major cause of maternal and neonatal morbidity and mortality. Preeclampsia usually manifests after the 20th week of gestation and is unpredictable in onset and progression. Main symptoms are proteinuria (>300 mg/24h), hypertension (newly occurred after 20. week of gestation is completed) and oedema. There is no specific diagnostic test available for clinical use. A multifactorial differential analysis of serum protein using mass spectrometry distinguished samples from pregnant women with severe early-onset preeclampsia (n=11) from those of control individuals with uneventful pregnancy (n=13). Serum proteins were fractionated by either their affinities to reversed-phase material coated magnetic beads or by fractionated precipitation. The on-average most abundant ion signals were observed at m/z 9390, m/z 9103 and m/z 8886. The best differentiating ion signals between the two sample groups were found at m/z 13,715, m/z 13,834 and m/z 13,891. The normalized intensities of these ion signals were on-average lower in the preeclampsia group than in the control group. The six ion signal intensities enabled sorting of the individual spectra with high accuracy. Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) analysis showed that a protein band migrating just above the 14 kDa marker band contained transthyretin. Densitometric analysis of the transthyretin bands showed lower intensities in the preeclampsia samples with respect to those of the controls. Nephelometric analysis of the serum samples determined the mean concentration of transthyretin in the preeclampsia group were lower than that in the control group, substantiating the role of transthyretin concentration differences in the comparison of the two groups. Altogether, our findings support the theory of preeclampsia being a heterogeneous disorder that might be sub-classified by a defined proteome signature in maternal blood using multifactorial analysis of affinity-fractionated serum samples.

Grantor dc:publisher

Publikationsserver der RWTH Aachen University

Year dc:date

2012

Author dc:creator

• Seidenspinner, Franka Nadine

Contributors dc:contributor

• Rath, Werner

• info:eu-repo/classification/ddc/610
• MALDI-MS
• Proteinmuster
• Präeklampsie
• Medizin
• early-onset Präeklampsie
• Serumanalyse
• affinity-mass spectrometry
• preeclampsia
• multifactorial analysis
• serum protein

Statement dc:rights

• info:eu-repo/semantics/openAccess

Language dc:language

ger

Identifier

https://publications.rwth-aachen.de/search?p=id:%22RWTH-CONV-124507%22

OAI identifier oai:identifier

oai:publications.rwth-aachen.de:63045