Die Anwendung des EVEIT-Tests in der Bewertung von Chemikalien und Medikamenten

With the objective of replacing the need of living animals to test chemicals respectively their reactions on the eye we created the EVEIT System. Therefore we designed a perfusion chamber in which rabbit corneas of animals that were killed for the food industry were clamped. Their nutrition was guaranteed by a special nutrition medium and the whole setup was stored in a breeder which ensured a constant temperature of 31°C. We did testings in mechanical and chemical ways on these cultivated corneas besides untreated control groups. The corneas were evaluated by morphological (endothelial and epithelial examinations) and biochemical (pH, glucose-lactate-metabolism, cytokines, growth factors in the medium) criteria. We could show that in principle a total healing of the cornea and a recover of the glucose-lactate-metabolism is possible after trauma. Different chemicals presented varying patterns of trauma and wound healing. The testings with different concentrations of the chemicals give at least a hint of dose response. But further testings with bigger groups of treated corneas and other concentrations need to be done to give better statements.

Grantor dc:publisher

Publikationsserver der RWTH Aachen University

Year dc:date

2012

Author dc:creator

• Goß, Miriam

Contributors dc:contributor

• Schrage, Norbert

• info:eu-repo/classification/ddc/610
• Hornhaut
• Verätzung
• Tierversuch
• Ex vivo
• Draize-Test
• Medizin
• cornea
• chemical burn
• Draize test
• animal experiment

Statement dc:rights

• info:eu-repo/semantics/openAccess

Language dc:language

eng

Identifier

https://publications.rwth-aachen.de/search?p=id:%22RWTH-CONV-124494%22

OAI identifier oai:identifier

oai:publications.rwth-aachen.de:63032