Publikationsserver der RWTH Aachen University
Biokompatibilität einer Kollagen-Typ-I-Membran im subretinalen Raum
Abstract
dc:descriptionThe in-vivo study investigated in rabbits after a maximum of 16 weeks, the integration and degradation of a collagen membrane in the subretinal space. The membrane could be successfully cultured with retinal pigment epithelium and where the inner collagen layer of Bruch's membrane, which consists mainly of type I collagen. The subretinal implantation of pigment epithelial cells is considered a therapeutic option for the reconstruction of pathological retinal pigment epithelium, which is especially submacular changed in patients with age-related macular degeneration. This dysfunction leads to degeneration of the photoreceptors and thus to a strong reduction of central vision. Since studies have shown that pigment epithelial cells transplanted in suspension do not form a layer of cells it is necessary that groups of cells are transplanted on a supporting tissue. In the selection of the supporting tissue biocompatibility plays a crucial role containing the degradation rate, size and shape. The collagen membrane showed excellent mechanical tissue compatibility with a thickness of 7μm and as 2mm squares. Through its autostability it could be simply prepared and was placed intraoperatively by a 0.9 mm sclerotomy to the subretinal space. The membrane was macroscopically and microscopically detectable in all observation periods. It showed up to 16 weeks postoperatively, an intact and shallow integration. A degradation in the form of fibrous, loosened areas was to describe from the 5th weeks and predominated in the 16th Week. At no time was signs of an acute or chronic inflammatory response found in the transplantation area. By the implantation of pure supporting tissue without pigment epithelial, photoreceptor cells could not be obtained. This was observed from day 7 postoperatively by shortened photoreceptor outer segments and a reduced number of cells in the outer nuclear cell layer. This was also visible with the synaptophysin antibody dye. GFAP-antibodies labeled 5 weeks after surgery all Müllercells and astrocytes of the nerve fiber layer, indicating an increase of intermediate filaments. The present studies have demonstrated that the collagen type I membrane has excellent biocompatibility through its degradation rate, size and shape and is a promising supporting tissue for the reconstruction of the union from retinal pigment epithelium and Bruch's membrane.
Degree
thesis:*- Grantor dc:publisher
- Publikationsserver der RWTH Aachen University
- Year dc:date
- 2011
Author and committee
dc:creator, dc:contributor.*- Author dc:creator
-
- Gaebler, Andrea
- Contributors dc:contributor
-
- Thumann, Gabriele
Subjects
dc:subject × 9Rights
dc:rights- Statement dc:rights
-
- info:eu-repo/semantics/openAccess
- Language dc:language
- ger
Identifiers
dc:identifier.*- OAI identifier oai:identifier
- oai:publications.rwth-aachen.de:62802