Fall-Kontrollstudie zur Bedeutung von Polymorphismen im MnSOD-Gen für die Sensibilisierung auf para-Phenylendiamin (PPD)

Allergic contact dermatitis is a hypersensitivity reaction of the skin which is caused by a combination of environmental factors and individual susceptibility. The reason why one individual develops an allergic contact dermatitis whereas others are not affected even under long term allergen exposure is not completely understood. The dye para-phenylene diamine (PPD) is a very common and potent contact sensitizer in man. It has been found that oxidative stress from reactive oxygen species (ROS) may play an important role in the pre-immunological phase of allergic contact dermatitis to PPD. Manganese superoxide dismutase (MnSOD) is one of the primary enzymes that directly scavenge potential harmful oxidizing species. Several genetic polymorphisms in the MnSOD gene have been associated with various disease risks. A single nucleotide exchange from cytosine to thymine at position 47 in the mitochondrial targeting sequence in exon 2 results in an amino acid exchange at position -9 in the signal peptide from alanin to valine (Ala-9Val). The same nucleotide exchange at nucleotide position 339 in exon 3 results in an amino acid exchange at position 58 from threonine to isoleucin (Ile58Thr). The aim of this case-control study was to establish suitable methods for detection of polymorphisms Ala-9Val and Ile58Thr and to investigate their possible association with contact sensitization to PPD in humans.The study was performed in 165 unrelated Caucasian cases and 202 age- and gender matched Caucasian controls. As no heterozygous (CT) or homozygous carrier (CC) for Ile58Thr was found, an association of this polymorphism with contact sensitization to PPD can be excluded. For Ala-9Val no statistically significant differences for genotypes and allelic frequencies between cases and controls (p > 0.05) were detected. However, there was an increased number of persons homozygous for the cytosine allele (Ala) among cases (27.4% vs. 22.9%, OR = 1.3). This was especially evident for female cases (28.9% vs. 22.9%, OR = 1.3), subjects older than 45 years (26.4% vs. 21.0%, OR = 1.3) and female cases older than 45 years (24.5% vs. 17.9%, OR = 1.5). Although this polymorphism apparently has no strong impact on the individual susceptibility to develop a sensitization to PPD, these results may show an increased risk for females, especially of higher age.

Grantor dc:publisher

Publikationsserver der RWTH Aachen University

Year dc:date

2005

Author dc:creator

• Brans, Richard

Contributors dc:contributor

• Blömeke, Brunhilde

• info:eu-repo/classification/ddc/610
• Medizin
• allergische Kontaktdermatitis
• para-Phenylendiamin
• Mangansuperoxiddismutase
• Polymorphismus
• Suszeptibilität
• allergic contact dermatitis
• para-phenylene diamine
• manganese superoxide dismutase
• polymorphism
• susceptibility

Statement dc:rights

• info:eu-repo/semantics/openAccess

Language dc:language

ger

Identifier

https://publications.rwth-aachen.de/search?p=id:%22RWTH-CONV-123789%22

OAI identifier oai:identifier

oai:publications.rwth-aachen.de:62205