Abhängigkeit der Phagozytose, des Oxidativen Bursts und des Killings durch neutrophile Granulozyten von Melanin und Carotenoiden bei Exophiala dermatitidis

The black melanized yeast Exophiala dermatitidis is a potentially pathogenic fungus frequently isolated from patients suffering from phaeohyphomycosis. Whereas the synthesis and the redox properties of melanin have been studied intensively, the influence of melanin and carotenoids on the phagocytosis, the oxidative burst and the killing of Exophiala dermatitidis by human neutrophils has not been studied. Using a combination of flow cytometrie, epifluorescence interference contrast microscopy and a colonie-count-dependent method their effects on these phenomena were analyzed. Exophiala dermatitidis wild-type strain Emmons 8656 and several melanin-deficient mutants were applied for this. The results show that melanin do not influence the phagocytosis or the oxidative burst of the neutrophils, but that melanin prevents this pathogen fungus from being killed in the phagolysosome of the neutrophils. The carotenoids torulene and torularhodine were not found to contribute to the prevention of killing. The ability of the melanized strains of Exophiala dermatitidis to block the effects of the neutrophils oxidative burst may play an important role in the pathogenesis of infections with these fungus.

Grantor dc:publisher

Publikationsserver der RWTH Aachen University

Year dc:date

2004

Author dc:creator

• Schauerte, Nicole Andrea

Contributors dc:contributor

• Schnitzler, Norbert

• info:eu-repo/classification/ddc/610
• Medizin

Statement dc:rights

• info:eu-repo/semantics/openAccess

Language dc:language

ger

Identifier

https://publications.rwth-aachen.de/search?p=id:%22RWTH-CONV-123619%22

OAI identifier oai:identifier

oai:publications.rwth-aachen.de:62021