Immunhistochemischer Nachweis des Retinoblastomgenproduktes und seine Bedeutung als Prognosefaktor bei Patientinnen mit primärem Mammakarzinom

Retinoblastoma gene is the prototype of tumor suppressor gene which controls cell cycle at the G1 phase. The expression of retinoblastoma gene product (Rb protein) was studied by immunohistochemical analysis of 101 cases of breast cancer over a retrospective period of 12 years. The prognostic force of expression of Rb protein was compared with established prognostic parameters (TNM-classification, lymph node status, steroid receptor status, histological grade). Monoclonal antibody (IgG1, G3-245, Biermann) in conjugation with the indirect Avidin-Biotin-immunoperoxidase technique was used for immunolocalization of Rb protein. Specific immunoactivity was scored on the basic of the Remmele IRS (immunoreactive score). Each case was assessed independently by two observers. In the univariate survival analysis was first demonstrated the significant association of the patients survival time with histological staging (pTNM=0.0011, pT=0.0238, pN= 0.0008, pM=0.0098) and the histological grade (p=0.0045). Studying the validity of the oestrogen and progesterone receptor status, only the progesterone receptor status showed a statistical significance (p=0.021), which is absolutely in agreement with the consent of St. Gallen 1998. There was no significant correlation between Rb gene expression and the survival. The percentage of positive cells (RbPP) showed a significant relationship to histological grade (p=0.0275). Correlation between Rb protein and the other clinical parameters was not found. In a following Cox multivariate analysis the established histological parameters like nodal status (p=0.0002), progesterone receptor status (p= 0.0076) and tumor size (p=0.0110) were confirmed as independent predictor of patient outcome, unlike Rb protein. The results show that the expression of Rb protein had no prognostic value in univariate or multivariate analysis.

Grantor dc:publisher

Publikationsserver der RWTH Aachen University

Year dc:date

2006

Author dc:creator

• Fischer, Nele

Contributors dc:contributor

• Biesterfeld, Stefan

• info:eu-repo/classification/ddc/610
• Medizin
• Brustkrebs
• Prognose
• Langfristige Prognose
• Mammakarzinom
• Retinobastomgen
• Immunhistochemie
• Prognosefaktor
• breast cancer
• retinoblastom gene
• immunolocalization
• prognostic parameter

Statement dc:rights

• info:eu-repo/semantics/openAccess

Language dc:language

ger

Identifier

https://publications.rwth-aachen.de/search?p=id:%22RWTH-CONV-123262%22

OAI identifier oai:identifier

oai:publications.rwth-aachen.de:61619