Fetuin-A als Risikomarker bei Patienten mit Niereninsuffizienz : Nephelometrie als neues Nachweisverfahren bei Risikokollektiven

The cardiovascular mortality rate in the dialysis population is dramatically increased compared to the population without renal failure. This increased mortality rate occurs because of so called "accelerated artherosclerosis", which is partially attributed to the increased calcium-phosphate-product and hyperphosphatemia in the serum. The calcification inhibiting protein ahsg/fetuin-a accounts for 50% of the precipitation inhibitory effect in serum and acts in vivo and in vitro. Additionally ahsg/fetuin-a is a negative acute phase protein whose concentration is downregulated during acute or chronic inflammation. In this context it has recently been shown that the ashg/fetuin-a concentration is significantly decreased in the dialysis population in contrast to non-renal patients correlating with a higher risk of cardiovascular and overall mortality. A decreased ahsg/fetuin-a concentration in serum is now considered to be a new important cardiovascular risk factor in the dialysis population.In the past the detection of ahsg/fetiun-a was achieved via ELISA (enzyme linked immuno sorbent assay), which due to the high dilution factors involved has been shown to be very inaccurate. By developing nepholomerty to measure ahsg/fetuin-a as outlined in this paper we have successeded to establish a robust risk factor screening methodology.Nephelometry was established in the following steps:1.determination of an appropriate antibody2.setting the optimal level of dilution3.selection of the appropiate standard serum4.vertification of the results with westenblot analysis5.exemplarly measurement of control groupsNephelometry has been proven to be a very stable measuring technique, which can be performed with a significantly higher accuracy than the previously used ELISA test. It is less time consuming (one sample can be measured in 5 minutes) and due to the much lower level of dilutions it is also less susceptible to inaccuracy. In the future it would be useful to transform the nepholometry test to an automized machine that can measure multiple samples at once instead of doing single sample measurements as undertaken in this study.

Grantor dc:publisher

Publikationsserver der RWTH Aachen University

Year dc:date

2006

Author dc:creator

• Koll, Anne

Contributors dc:contributor

• Ketteler, Markus

• info:eu-repo/classification/ddc/610
• Medizin
• Dialyse
• Arteriosklerose
• Chronische Niereninsuffizienz
• Ahsg/Fetuin-A
• cardiovaskulärer Risikofaktor
• Nephelometrie
• cardiovascular risc factor
• nephelomerty
• renal failure
• dialysis

Statement dc:rights

• info:eu-repo/semantics/openAccess

Language dc:language

ger

Identifier

https://publications.rwth-aachen.de/search?p=id:%22RWTH-CONV-122995%22

OAI identifier oai:identifier

oai:publications.rwth-aachen.de:61321