Lungenmorphologie und Apoptose nach partieller Liquidventilation im neonatalen Tiermodell

Partial liquid ventialtion is an alternative method to treat neonates suffering from respiratory insufficiency, as it improves gas exchange and pulmonary mechanics. For clinical application the histopathological changes are of high relevance, too. Apoptosis is an important component of the acute lung injury in newborns. After inducing lung injury with repeated saline lavages, nineteen newborn piglets were randomized in three groups: CV (conventional ventilation), CV+S (conventional ventilation with surfactant substitution) and PLV (partial liquid ventilation with FC-77). Additionally, one control group without induced lung injury was ventilated conventionally. In a further setting two perfluorocarbons were compared: a 6-hour-period of partial liquid ventilation with FC-77 (n=6) or POB (n=5) was followed by 18 hours of conventional ventilation. After 24 hours the lungs were removed for histopathologic examination and for TUNEL-assay studies to detect apoptotic nuclei. Histologic lung injury was lowest in the PLV-group. Compared with POB, lung injury after FC-77 treatment was significant reduced. Apoptotic nuclei were found in all groups, which did not show statistically significant differences. With regard to the histopathological findings, partial liquid ventilation is a promising new method of artificial ventilation. Hereby FC-77 may be superior to POB. Both perfluorocarbons did not enhance programmed cell death. Decreased lung damage after PLV is not caused by a lower rate of apoptosis.

Grantor dc:publisher

Publikationsserver der RWTH Aachen University

Year dc:date

2004

Author dc:creator

• Berkemeier, Eva Gesine

Contributors dc:contributor

• Hörnchen, Helmut

• info:eu-repo/classification/ddc/610
• Medizin
• Apoptose
• Liquidventilation
• Flüssigkeitsbeatmung
• Surfactantmangel
• Bronchopulmonale Dysplasie
• Atemnotsyndrom

Statement dc:rights

• info:eu-repo/semantics/openAccess

Language dc:language

ger

Identifier

https://publications.rwth-aachen.de/search?p=id:%22RWTH-CONV-121482%22

OAI identifier oai:identifier

oai:publications.rwth-aachen.de:59721