Mikrofluidische Strukturen für biochemische Analysen

The immunoassay is an important serological detection method for biochemical active materials (antigens / anti-bodies). By designing appropriate micro fluidic structures to perform the classic process steps of an immunoassay it can be integrated on a microfluidic chip. The reagents are transported through a micro fluidic reaction chamber where they perform the biochemical reactions. These reagents are stored in the micro-fluidic channels on the chip. The suitability of adhesive foils as low priced and simple seals of the microfluid channels is shown by performing immunoassays in micro-fluid cuvette structures. Signal detection can be performed by fluorescence and by chemoluminescence with sufficient accuracy for, e.g. the diagnosis of a cardiac infarct. A micro pump with two dimensional valve structures without moving parts was developed for the liquid-handling on the micro-fluidic chip. This pump can be manufactured by photolithographic techniques for prototyping as well as by mass production processes, like hot embossing and injection moulding. Integration of this micro pump into fluidic chip structures manufactured from SU-8 photoresist shows the suitability of this pump for the production of micro-fluid analysis systems. A computer control was developed, which can operate the complete process cycle for a micro-fluidic Immunoassay. The performance of the micro pump, and the reaction chamber were optimized by computer simulations. The channel crossing where the different reagents enter the reaction chamber was also improved by computational fluid dynamics (cfd). The performance of a myoglobin immunoassay in a completely integrated fluidic chip manufactured from PMMA shows the capability of detection of a myoglobin concentration of less than 100 ng/m. The measurement of these myoglobin concentrations can be used for the detection of cardiac infarcts from human blood samples.

Grantor dc:publisher

Publikationsserver der RWTH Aachen University

Year dc:date

2004

Author dc:creator

• Schlüter, Michael

Contributors dc:contributor

• Mokwa, Wilfried

• info:eu-repo/classification/ddc/570
• Biowissenschaften, Biologie

Statement dc:rights

• info:eu-repo/semantics/openAccess

Language dc:language

ger

Identifier

https://publications.rwth-aachen.de/search?p=id:%22RWTH-CONV-121326%22

OAI identifier oai:identifier

oai:publications.rwth-aachen.de:59548