Publikationsserver der RWTH Aachen University
Vergleichende Untersuchungen der Hämokompatibilität unterschiedlicher Polymere (Polypropylen, Cuprophan, PDLLA, PDLLA/HEMA) im Hinblick auf Thrombozytenadhäsion und -aktivierung
Abstract
dc:descriptionThe usage of biomaterials in modern medicine is of increasing importance. Biocompatibility is one of the most important parameters in their practical use. In case of direct blood contact haemocompatibility is of great importance. In this study four biopolymers (polypropylene, cuprophane (non-resorbable) and PDLLA, PDLLA/HEMA (resorbable)) are compared with regard to their haemocopatibility and suitability as an intravascular implant. The biomaterials are first incubated with isolated, discoid and non-activated platelets, drawn from fresh human blood. Paraformaldehyde 0,5% is used for fixation. Following a qualitative and quantitative detection of adherent and activated platelets on the used biomaterials by two anti-platelet antibodies, isolated from chicken. Results are compared with the qualitative and quantitative detection of adherent and activated platelets on the biomaterials by a common commercial anti-platelet antibody (CD 41). Immune fluorescence microscopy is used to proove the results. Due to the results the biopolymers are divided into the Goodmann-classification, which is used to quantify different levels of activation in platelets. Polypropylene as a material with a well-known high thrombogenity is used as a reference material. The two chicken-anti-platelet antibodies show nearly the same results on every biomaterial tested. They show a higher thrombogenity for polypropylene and cuprophane compared to the results with the CD 41 antibody. In contrast to this fact the results for PDLLA with the chicken-anti-platelet antibodies show a lower thrombogenity compared to the results with the CD 41 antibody. Results for PDLLA/HEMA are identically for every used antibody. There is are significant improvement in haemocompatibility of PDLLA/HEMA in comparison to PDLLA due to the addition of HEMA. PDLLA/HEMA and cuprophane show the lowest thrombogenity among the tested biopolymers. These results confirm PDLLA/HEMA as a potential candidate for the use as an intravascular implant.
Degree
thesis:*- Grantor dc:publisher
- Publikationsserver der RWTH Aachen University
- Year dc:date
- 2003
Author and committee
dc:creator, dc:contributor.*- Author dc:creator
-
- Keil, Jürgen
- Contributors dc:contributor
-
- Dahl, Jürgen vom
Subjects
dc:subject × 8Rights
dc:rights- Statement dc:rights
-
- info:eu-repo/semantics/openAccess
- Language dc:language
- ger
Identifiers
dc:identifier.*- OAI identifier oai:identifier
- oai:publications.rwth-aachen.de:59462