Publikationsserver der RWTH Aachen University
Untersuchungen zur Gefriertrocknung biologischer Zellen am Beispiel menschlicher Erythrozyten
Abstract
dc:descriptionFreeze-drying is a state of the art process for stabilization of pharmaceutical or biotechnological products. However, a successful preservation of living cells by freeze-drying is still a big challenge. This is mainly due to increased requirements on the product quality, since even smallest structural changes of the complex cell morphology can lead to a loss of function or viability. Using human erythrocytes as a simple model for biological cells in general, several mechanisms were investigated presumed to be responsible for cell damage during the freeze-drying process. Besides a considerable progress in understanding of these mechanisms, the cell recovery rate could gradually be increased from about 0% to 50% by means of following major developments and studies. A new low-temperature freeze-drying device was designed and manufactured that allows a controlled cooling of condenser and shelf down to >190°C and >80°C, respectively. A variation of shelf temperature and drying time led to optimized vacuum-drying conditions. The temperature inside the sample, which was either measured or calculated, was found to be a key quantity for the resulting cell damage. A cooling rate variation showed that ultra rapid cooling with a rate of up to 13.500 K/min leads to amorphous sample structures providing best cell protection during subsequent drying.
Degree
thesis:*- Grantor dc:publisher
- Publikationsserver der RWTH Aachen University
- Year dc:date
- 2000
Author and committee
dc:creator, dc:contributor.*- Author dc:creator
-
- Rindler, Volker
- Contributors dc:contributor
-
- Rau, Günter
Subjects
dc:subject × 7Rights
dc:rights- Statement dc:rights
-
- info:eu-repo/semantics/openAccess
- Language dc:language
- ger
Identifiers
dc:identifier.*- OAI identifier oai:identifier
- oai:publications.rwth-aachen.de:59422